Synopsis "Cardiac Gene Therapy: Methods and Protocols (in English)"
Part I Overview 1. Updates on Cardiac Gene Therapy Research and Methods: Overview of Cardiac Gene Therapy Francisco J. Romeo, Spyros A. Marvopoulos, and Kiyotake Ishikawa Part II Gene Suppression, Editing, and Reprogramming 2. Tough Decoy-Mediated Cardiac Gene Suppression Changwon Kho 3. Direct Reprogramming of Adult Human Cardiac Fibroblasts into Induced Cardiomyocytes using miRcombo Camilla Paoletti, Carla Divieto, and Valeria Chiono 4. CRISPR/Cas9 Gene Editing of RYR2 in Human iPSC Derived Cardiomyocytes to Probe Ca2+ Signaling Aberrancies of CPVT Arrhythmogenesis Naohiro Yamaguchi, Xiao-Hua Zhang, and Martin Morad 5. Enhancing Cardiomyocyte Transcription using In Vivo CRISPR/Cas9 Systems Eric Schoger and Laura C. Zelarayán 6. AAV Mediated Somatic Gene Editing for Cardiac and Skeletal Muscle in a Large Animal Model Tilman Ziegler, Tarik Bozoglu, and Christian Kupatt Part III Cardiac Gene Therapy Vectors and Promotors 7. Optimization of Synthesis of Modified mRNA Jimeen Yoo and Lior Zangi 8. Design and Production of Heart Chamber-Specific AAV9 Vectors Alina S. Bilal, Donna J. Thuerauf, Erik A. Blackwood, and Christopher C. Glembotski 9. Generation of Atrial-Specific Construct using Sarcolipin Promoter Associated CRM4 Enhancer Dongtak Jeong Part IV Cardiac Gene Delivery Methods 10. Cardiac Targeted Adeno-Associated Virus Injection in Rats Michael G. Katz, Yoav Hadas, Adam S. Vincek, Nataly Shtraizent, Eric Schadt, and Efrat Eliyahu 11. Cardiac Gene Delivery in Large Animal Models-Antegrade Techniques Spyros A. Mavropoulos, Kelly P. Yamada, Tomoki Sakata, and Kiyotake Ishikawa 12. Locked Nucleic Acid AntimiR Therapy for the Heart Sabine Samolovac and Rabea Hinkel 13. Cardiac Gene Delivery in Large Animal Models: Selective Retrograde Venous Injection Philipp Schlegel and Philip W. J. Raake 14. Endocardial Gene Delivery using NOGA Catheter System Satu Siimes, Niko Järveläinen, Henna Korpela, and Seppo Ylä-Herttuala 15. Surgical Methods for Cardiac Gene Delivery in Large Animals Michael G. Katz, Yoav Hadas, Adam S. Vincek, Nataly Shtraizent, Hylton P. Gordon, Peter Pastuszko, Eric Schadt, and Efrat Eliyahu 16. Atrial Gene Painting in Large Animal Model of Atrial Fibrillation Weilan Mo and J. Kevin Donahue 17. Stent-Based Gene Delivery for Coronary Disease Ivan S. Alferiev, Michael Chorny, Robert L. Wilensky, Robert J. Levy, and Ilia Fishbein 18. Selective Anti-AAV Antibody Depletion by Hemapheresis and Immunoadsorption Alejandro Orlowski and Thomas Weber 19. Ex Vivo Delivery of Viral Vectors by Organ Perfusion for Cardiac Transplantation Gene Therapy Michelle Mendiola Pla, Amy Evans, Paul Lezberg, and Dawn E. Bowles Part V Pulmonary Hypertension 20. Intra-Airway Gene Delivery for Pulmonary Hypertension in Rodent Models Malik Bisserier, Olivier Boucherat, Sebastien Bonnet, and Lahouaria Hadri &nb
