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portada micrornas as tools in biopharmaceutical production (in English)
Type
Physical Book
Illustrated by
Publisher
Author
Year
2012
Language
Inglés
Pages
126
Format
Hardcover
Dimensions
23.4 x 16.0 x 1.5 cm
Weight
0.41 kg.
ISBN
9400751273
ISBN13
9789400751279
Categories

micrornas as tools in biopharmaceutical production (in English)

Niall Barron (Illustrated by) · Springer · Hardcover

micrornas as tools in biopharmaceutical production (in English) - Barron, Niall

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Synopsis "micrornas as tools in biopharmaceutical production (in English)"

Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.

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